c-pace ep culture stimulation system Search Results


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IonOptix c-pace em stimulator
C Pace Em Stimulator, supplied by IonOptix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IonOptix cell culture stimulator c-pace ep
Cell Culture Stimulator C Pace Ep, supplied by IonOptix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IonOptix electrical pulse stimulation c-pace ep
Response to electrical pulse <t>stimulation</t> (EPS) in L6, C2C12, and human primary myotubes. A : mRNA levels of myosin and actin isoforms. B : representative images of myotubes during proliferation and after complete differentiation. Staining for desmin and myosin was performed using specific antibodies as described in methods . Representative images are shown for each staining. C : protein content per well measured by bicinchoninic acid assay. Box-and-whisker Tukey plot; n totals are as follows: HSMC: n = 11 replicates from 11 independent donors, C2C12: n = 7 independent experiments, L6: n = 6 independent experiments, Kruskal-Wallis test with Dunn’s multiple comparison. D–F : mRNA levels of the GTPase-activating protein for Rab family protein TBC1D1 , Ca 2+ /calmodulin-dependent protein kinase II ( CAMK2A ), and RAC1 . G : electrical pulse stimulation-induced glucose uptake measured using 2-[1,2- 3 H]deoxy- d -glucose. Mean ± SE and individual data points: n totals are as follows: HSMC: n = 7 replicates from 7 independent donors, C2C12: n = 6 independent experiments, L6: n = 7 independent experiments, one-way ANOVA with Tukey’s multiple comparison. H and I : mRNA expression of exercise-responsive genes measured by quantitative PCR. Box-and-whisker Tukey plot; n totals are as follows: HSMC: n = 5 replicates from 5 independent donors, C2C12: n = 6 independent experiments, L6: n = 6 independent experiments, two-way ANOVA with Fisher’s least-significant difference comparison. # P < 0.05, significant response to EPS. * P < 0.05. HSMC, human skeletal muscle cells.
Electrical Pulse Stimulation C Pace Ep, supplied by IonOptix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IonOptix c-pace ep cell culture stimulator cep100
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C Pace Ep Cell Culture Stimulator Cep100, supplied by IonOptix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IonOptix spheroid electrical stimulation culture pacing system c-pace em
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Spheroid Electrical Stimulation Culture Pacing System C Pace Em, supplied by IonOptix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IonOptix c-pace em culture stimulation system
Table of Materials
C Pace Em Culture Stimulation System, supplied by IonOptix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IonOptix field culture stimulator c-pace ep
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Field Culture Stimulator C Pace Ep, supplied by IonOptix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IonOptix 8-channel c-pace cell culture stimulator
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8 Channel C Pace Cell Culture Stimulator, supplied by IonOptix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IonOptix c-pace ep culture stimulation system
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C Pace Ep Culture Stimulation System, supplied by IonOptix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IonOptix c-pace bulk culture stimulator
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C Pace Bulk Culture Stimulator, supplied by IonOptix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IonOptix c-dish electrode powered by a c-pace ep cell culture stimulator
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C Dish Electrode Powered By A C Pace Ep Cell Culture Stimulator, supplied by IonOptix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Response to electrical pulse stimulation (EPS) in L6, C2C12, and human primary myotubes. A : mRNA levels of myosin and actin isoforms. B : representative images of myotubes during proliferation and after complete differentiation. Staining for desmin and myosin was performed using specific antibodies as described in methods . Representative images are shown for each staining. C : protein content per well measured by bicinchoninic acid assay. Box-and-whisker Tukey plot; n totals are as follows: HSMC: n = 11 replicates from 11 independent donors, C2C12: n = 7 independent experiments, L6: n = 6 independent experiments, Kruskal-Wallis test with Dunn’s multiple comparison. D–F : mRNA levels of the GTPase-activating protein for Rab family protein TBC1D1 , Ca 2+ /calmodulin-dependent protein kinase II ( CAMK2A ), and RAC1 . G : electrical pulse stimulation-induced glucose uptake measured using 2-[1,2- 3 H]deoxy- d -glucose. Mean ± SE and individual data points: n totals are as follows: HSMC: n = 7 replicates from 7 independent donors, C2C12: n = 6 independent experiments, L6: n = 7 independent experiments, one-way ANOVA with Tukey’s multiple comparison. H and I : mRNA expression of exercise-responsive genes measured by quantitative PCR. Box-and-whisker Tukey plot; n totals are as follows: HSMC: n = 5 replicates from 5 independent donors, C2C12: n = 6 independent experiments, L6: n = 6 independent experiments, two-way ANOVA with Fisher’s least-significant difference comparison. # P < 0.05, significant response to EPS. * P < 0.05. HSMC, human skeletal muscle cells.

Journal: American Journal of Physiology - Cell Physiology

Article Title: Comparative profiling of skeletal muscle models reveals heterogeneity of transcriptome and metabolism

doi: 10.1152/ajpcell.00540.2019

Figure Lengend Snippet: Response to electrical pulse stimulation (EPS) in L6, C2C12, and human primary myotubes. A : mRNA levels of myosin and actin isoforms. B : representative images of myotubes during proliferation and after complete differentiation. Staining for desmin and myosin was performed using specific antibodies as described in methods . Representative images are shown for each staining. C : protein content per well measured by bicinchoninic acid assay. Box-and-whisker Tukey plot; n totals are as follows: HSMC: n = 11 replicates from 11 independent donors, C2C12: n = 7 independent experiments, L6: n = 6 independent experiments, Kruskal-Wallis test with Dunn’s multiple comparison. D–F : mRNA levels of the GTPase-activating protein for Rab family protein TBC1D1 , Ca 2+ /calmodulin-dependent protein kinase II ( CAMK2A ), and RAC1 . G : electrical pulse stimulation-induced glucose uptake measured using 2-[1,2- 3 H]deoxy- d -glucose. Mean ± SE and individual data points: n totals are as follows: HSMC: n = 7 replicates from 7 independent donors, C2C12: n = 6 independent experiments, L6: n = 7 independent experiments, one-way ANOVA with Tukey’s multiple comparison. H and I : mRNA expression of exercise-responsive genes measured by quantitative PCR. Box-and-whisker Tukey plot; n totals are as follows: HSMC: n = 5 replicates from 5 independent donors, C2C12: n = 6 independent experiments, L6: n = 6 independent experiments, two-way ANOVA with Fisher’s least-significant difference comparison. # P < 0.05, significant response to EPS. * P < 0.05. HSMC, human skeletal muscle cells.

Article Snippet: Differentiated myotubes were subjected to electrical pulse stimulation (EPS; C-Pace EP; Ionoptix, MA) at 40 V, 2 ms, 1 Hz during 3 h in low-glucose DMEM.

Techniques: Staining, Acid Assay, Whisker Assay, Comparison, Expressing, Real-time Polymerase Chain Reaction

Table of Materials

Journal: Journal of visualized experiments : JoVE

Article Title: Slicing and Culturing Pig Hearts under Physiological Conditions

doi: 10.3791/60913

Figure Lengend Snippet: Table of Materials

Article Snippet: C-Pace EP Cell Culture Stimulator , Ion Optix , CEP100 , .

Techniques: Cell Culture, Sterility, Recombinant