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Image Search Results
Journal: American Journal of Physiology - Cell Physiology
Article Title: Comparative profiling of skeletal muscle models reveals heterogeneity of transcriptome and metabolism
doi: 10.1152/ajpcell.00540.2019
Figure Lengend Snippet: Response to electrical pulse stimulation (EPS) in L6, C2C12, and human primary myotubes. A : mRNA levels of myosin and actin isoforms. B : representative images of myotubes during proliferation and after complete differentiation. Staining for desmin and myosin was performed using specific antibodies as described in methods . Representative images are shown for each staining. C : protein content per well measured by bicinchoninic acid assay. Box-and-whisker Tukey plot; n totals are as follows: HSMC: n = 11 replicates from 11 independent donors, C2C12: n = 7 independent experiments, L6: n = 6 independent experiments, Kruskal-Wallis test with Dunn’s multiple comparison. D–F : mRNA levels of the GTPase-activating protein for Rab family protein TBC1D1 , Ca 2+ /calmodulin-dependent protein kinase II ( CAMK2A ), and RAC1 . G : electrical pulse stimulation-induced glucose uptake measured using 2-[1,2- 3 H]deoxy- d -glucose. Mean ± SE and individual data points: n totals are as follows: HSMC: n = 7 replicates from 7 independent donors, C2C12: n = 6 independent experiments, L6: n = 7 independent experiments, one-way ANOVA with Tukey’s multiple comparison. H and I : mRNA expression of exercise-responsive genes measured by quantitative PCR. Box-and-whisker Tukey plot; n totals are as follows: HSMC: n = 5 replicates from 5 independent donors, C2C12: n = 6 independent experiments, L6: n = 6 independent experiments, two-way ANOVA with Fisher’s least-significant difference comparison. # P < 0.05, significant response to EPS. * P < 0.05. HSMC, human skeletal muscle cells.
Article Snippet: Differentiated myotubes were subjected to electrical
Techniques: Staining, Acid Assay, Whisker Assay, Comparison, Expressing, Real-time Polymerase Chain Reaction
Journal: Journal of visualized experiments : JoVE
Article Title: Slicing and Culturing Pig Hearts under Physiological Conditions
doi: 10.3791/60913
Figure Lengend Snippet: Table of Materials
Article Snippet: C-Pace EP Cell Culture Stimulator ,
Techniques: Cell Culture, Sterility, Recombinant